PolyLC Inc., Columbia USA has developed unique materials and methods for HPLC of proteins and peptides. The polypeptide-based coatings permit the resolution of many mixtures not possible with other methods. PolyLC Inc.’s product line includes columns and cartridges for Hydrophobic Interaction Chromatography, Hydrophilic Interaction Chromatography, cation exchange of peptides, selective removal of SDS from peptide digests, anion-exchange of proteins, and HPLC of complex carbohydrates. PolyLC Inc. can offer technical support for the most difficult biochemical separations.
- PolyGLYCOPLEX – for complex carbohydrates
- PolyCAT A – for cation-exchange of proteins
- Hydrophilic Interaction Chromatography (HILIC)
- Size Exclusion Chromatography (SEC)
- PolySULFOETHYL Aspartamide – for cation-exchange of peptides
- PolyPROPYL-, PolyETHYL-, PolyMETHYL Aspartamide- for hydrophobic interaction chromatography (HIC) of proteins and peptides
- Getting rid of SDS
- PolyWAX LP – for anion-exchange of proteins
PolyLC do PRIONS
Recent outbreaks of bovine spongiform encephalopathy (BSE) or mad cow disease in Europe have highlighted the need to develop a method to identify pathogenic prion proteins in the blood of animals and humans.
In collaboration with Dr. Mary Jo Schmerr of the United States Department of Agriculture (USDA), PolyLC has helped to develop the first practical test for pathogenic prion proteins in blood. Experimentally, the test has given positive results in a variety of TSE (Transmissible Spongiform Encephalopathy) diseases, including:
- Scrapie (in sheep)
- Chronic wasting disease (in elk and mule deer)
and similar prion diseases in hamster, llama, chimpanzee and humans
At present the test can detect prion protein in the picogram range per ml. blood. This is sufficiently sensitive to give positive results in infected animals before clinical symptoms develop. The test has not yet been validated. Validation studies are in progress in the case of scrapie. That will require correlation of the positive results with the animals subsequently developing clinical cases of disease. This should take 1-3 more years. In the interim, all use of this test should be considered to be research rather than definitive diagnosis.
PolyLC’s HPLC columns of PolyHYDROXYETHYL Aspartamide permit the isolation of pure pathogenic prion protein from extracts from brain and other tissues of diseased animals. The protein is obtained free of detergent with recovery >90%, in a form that readily forms complexes with anti-prion antibodies
For more technical information contact Poly LC at: http://www.chromatechnologies.com
For Pricing, Availability and Ordering Information Contact: firstname.lastname@example.org
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